NEED HELP WITH BLANK BOX QUESTION
(question 1 and 4 in conclusion part. )
* It would be nice if you can check my answeres too.
NOTES:
Tube Sample Lane
1 1 kb DNA ladder 1
2 Mother’s DNA 2
3 Child’s DNA 3
4 A.F. #1 DNA 4
5 A.F. #2 DNA 5
Record the distance each ladder band moved from the well in mm along with the size of the DNA fragments in that band in bp units, based on the bp given in step 19 , in your Lab Notes.
Lane 1:
1,000 bp 34 mm
900 bp 36 mm
800 bp 38 mm
700 bp 40 mm
600 bp 43 mm
500 bp 47 mm
400 bp 51 mm
300 bp 56 mm
250 bp 60 mm
200 bp 64 mm
150 bp 69 mm
100 bp 77 mm
50 bp 90 mm
Lane 2:
37 mm 850bp
59 mm 265bp
Lane 3:
37 mm 850bp
44 mm 575bp
Lane 4:
41 mm 670bp
43 mm 600bp
Lane 5:
44 mm 575bp
55 mm 320bp
Experiment: Agarose Gel Electrophoresis of DNA Fragments
Lab Results
- List the distances traveled in mm for the bands in the DNA ladder in the table below.
Remember, smaller fragments travel farther than longer ones, so the top-most band will be the 1,000 bp sized DNA fragments whereas the bottom-most band will be the 50 bp sized DNA fragments.DNA Ladder Band Distance (mm) 50 bp 90 mm 100 bp 77 mm 150 bp 69 mm 200 bp 64 mm 250 bp 60 mm 300 bp 56 mm 400 bp 51 mm 500 bp 47 mm 600 bp 43 mm 700 bp 40 mm 800 bp 38 mm 900 bp 36 mm 1,000 bp 34 mm - Whose sample had the approximately 570 bp and 320 bp sized DNA fragments?
A.F. #2 - What were the sizes of the DNA fragments for alleged father #1?
41 mm 670bp 43 mm 600bp
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