| Table 2: Balloon Circumference vs. Temperature | |||
| Tube | Temperature (°C) | Balloon Circumference (Uninflated; cm) | Balloon Circumference (Final; cm) |
| 1 – (Cold) | |||
| 2 – (RT) | |||
| 3 – (Hot) |
Post-Lab Questions
1. What reaction is being catalyzed in this experiment?
2. What is the enzyme in this experiment? What is the substrate?
3. What is the independent variable in this experiment? What is the dependent variable?
4. How does the temperature affect enzyme function? Use evidence from your data to support your answer.
5. Draw a graph of balloon diameter vs. temperature. What is the correlation?
6. Is there a negative control in this experiment? If yes, identify the control. If no, suggest how you could revise the experiment to include a negative control.
7. In general, how would an increase in substrate alter enzyme activity? Draw a graph to illustrate this relationship.
8. Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would you find the enzymes for this experiment? What substrate would you use?