How are new molecules of DNA synthesized in living cells?

How are new molecules of DNA synthesized in living cells?:Agarose Gel Electrophoresis of DNA Fragments

Lab Results

1. List the distances traveled in mm for the bands in the DNA ladder in the table below.
   Remember, smaller fragments travel farther than longer ones, so the top-most band will be the 1,000 bp sized DNA fragments whereas the bottom-most band will be the 50 bp sized DNA fragments. 

   DNA Ladder
   Band	Distance (mm)
   50 bp	90 mm
   100 bp	77 mm
   150 bp	69 mm
   200 bp	64 mm
   250 bp	60 mm
   300 bp	56 mm
   400 bp	51 mm
   500 bp	47 mm
   600 bp	43 mm
   700 bp	40 mm
   800 bp	38 mm
   900 bp	36 mm
   1,000 bp	34 mm

    

2. Whose sample had the approximately 570 bp and 320 bp sized DNA fragments?
   

   A.F. #2

3. What were the sizes of the DNA fragments for alleged father #1?
   

   41 mm 670bp 43 mm 600bp

Data Analysis

1. Which size DNA fragment did the child inherit from her mother?
   

   37 mm 850bp

2. Which alleged father, if any, can be definitively ruled out as the child’s biological father?
   

   A.F. #2

Conclusions

1. How are new molecules of DNA synthesized in living cells?
   
2. What is the function of DNA?
   

   DNA has genetic information that controls our cells. So, DNA is like a blueprint that shows how to construct components of cells like proteins and ribonucleic acid (RNA). This information is carried down to newer generations through inheritance.

3. If each individual has such a small amount of DNA in their cells, how do the bands on the gel contain enough DNA to be visible?
   

   In order to make DNA visible. The Gel has to be soaked in a dye (ethidium bromide) to bind with the DNA and rinsed off after. Ethidium bromide helps to make DNA visible by glowing brightly in UV rays.

4. Humans only have a few eye colors and only four ABO-based blood types. How can DNA tests definitively identify individuals when many people have brown eyes or type A blood?
   
5. Suppose a suspicious hair was found in a victim’s home. A gel is set up with the DNA fragments of two suspected criminals in lanes 4 and 5, the DNA fragments of the suspicious hair in lane 3, and the victim’s DNA fragments, as a negative control, are in lane 2. A DNA ladder is in lane 1. The resulting gel is below. Which suspect, if any, committed the crime? Explain your answer.
   

   Lane 4,5 (two suspected criniminals)

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