Gram Staining Experiment Inventory

Gram Staining Experiment Inventory
Gram Staining Experiment Inventory

Gram Staining Experiment Inventory

Materials 2 “Gram” Slides from Experiment 1

Crystal Violet

Deionized Water

Gram Iodine

Decolorizer

Safranin

*Sink or Disposable Plastic Container

*10% Bleach Solution

Labware 1/2 Bibulous Paper Sheet

Parafilm®

1 Pair of Disposable Gloves

*Lab Notebook (optional)

*Scissors

Note: You must provide the materials listed in *red.

EXPERIMENT 3: GRAM STAINING

The Gram staining technique was invented by Dr. Hans Christian Gram (1853 – 1938), a scientist who worked in microbiology labs with Robert Koch. Gram staining was a major breakthrough in the identification and classification of bacteria; it distinguishes microbes broadly into Gram-positive and Gram-negative groups. These two groups can cause very different and important health consequences. Gram-positive bacteria have thick cell walls made up of many (up to 40) layers of peptidoglycan. This thick cell wall prevents microbes from losing the purple stain (upon destaining). Gram-negative bacteria have relatively thin cell walls with only a few layers of peptidoglycan and an outer cell membrane. Gram-negative bacteria are visualized with a counterstain that creates a reddish-pink color on the bacteria.

Place Your Order Here!

Leave a Comment

Your email address will not be published. Required fields are marked *