Biology

Introduction to Science Experiment 1 Simple Staining Materials Crystal Violet

Introduction to ScienceExperiment 1 Simple Staining Materials Crystal Violet 2 mL Deionized Water 6 Drops Sterile Saline Nutrient Agar Candle Matches *10% Bleach Solution *Isopropyl Alcohol *Sink or Disposable Plastic Container *Microwave or Boiling Water Bath *Permanent Marker Labware 100 mL Beaker Forceps 2 Sterile Cotton Swabs 1 Pair of Disposable Gloves Resusable Metal Inoculation […]

Introduction to Science Experiment 1 Simple Staining Materials Crystal Violet Read More »

Gram Staining Experiment Inventory

Gram Staining Experiment Inventory Materials 2 “Gram” Slides from Experiment 1 Crystal Violet Deionized Water Gram Iodine Decolorizer Safranin *Sink or Disposable Plastic Container *10% Bleach Solution Labware 1/2 Bibulous Paper Sheet Parafilm® 1 Pair of Disposable Gloves *Lab Notebook (optional) *Scissors Note: You must provide the materials listed in *red. EXPERIMENT 3: GRAM STAINING

Gram Staining Experiment Inventory Read More »

Use your sterile inoculating loop to transfer a colony from the culture plate that corresponds with the slide you just prepared,

Use your sterile inoculating loop to transfer a colony from the culture plate that corresponds with the slide you just prepared, and gently mix the microbes with the nigrosin. 4. Place the clean slide at about a 45° angle next to the nigrosin, and use the slide to gently spread the dye over the specimen

Use your sterile inoculating loop to transfer a colony from the culture plate that corresponds with the slide you just prepared, Read More »

NEGATIVE STAINING

NEGATIVE STAINING Negative staining provides a more detailed assessment of a microbe’s morphology and helps prevent any distortion of the mi- crobe’s ultrastructure by the staining procedure. It also makes the outline of the cells highly visible. Nigrosin and bacterial cells are negatively charged. Therefore, negative staining works because like charges repel each other, preventing

NEGATIVE STAINING Read More »

Introduction to ScienceExperiment 2Negative Staining Experiment Inventory

Introduction to ScienceExperiment 2Negative Staining Experiment Inventory Materials 2 “Negative slides” from Experiment 1 Nigrosin Candle Matches *Sink or Disposable Plastic Container *10% Bleach Solution *Isopropyl Alcohol Labware 1 Clean Microscope Slide Reusable Metal Inoculation Loop Parafilm® 1 Pair of Disposable Gloves *Lab Notebook (optional) *Scissors *Timer Note: You must provide the materials listed in

Introduction to ScienceExperiment 2Negative Staining Experiment Inventory Read More »

Why was the Gram iodine added to the Gram staining procedure?

Experiment 3 Post-Lab Questions 1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points) Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall. Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin). 2. What

Why was the Gram iodine added to the Gram staining procedure? Read More »

What different characteristic(s) exist between the two groups that account for the different staining conditions?

Experiment 3 Post-Lab Questions 1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points) Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall. Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin). 2. What

What different characteristic(s) exist between the two groups that account for the different staining conditions? Read More »

What color are the Gram-positive bacteria after Gram staining?

Experiment 3 Post-Lab Questions 1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points) Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall. Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin). 2. What

What color are the Gram-positive bacteria after Gram staining? Read More »

describe what physical/visual characteristics you were able to observe after performing the negative staining vs. after performing the simple stain.

Results Tables Table 2: Experiment 2 Staining Observations (5 points)     Stain used:   Nigrosin Observations: Background is stained, bacteria shows up as clear spiral.       Experiment 2 Post-Lab Questions 1. After visualizing the stained samples either using your microscope or by looking at the sample images provided, describe what physical/visual characteristics

describe what physical/visual characteristics you were able to observe after performing the negative staining vs. after performing the simple stain. Read More »