Biology

Why is a counterstain (safranin) added to the Gram staining procedure?

Experiment 3 Post-Lab Questions 1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points) Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall. Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin). 2. What […]

Why is a counterstain (safranin) added to the Gram staining procedure? Read More »

Why was the Gram iodine added to the Gram staining procedure?

Experiment 3 Post-Lab Questions 1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points) Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall. Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin). 2. What

Why was the Gram iodine added to the Gram staining procedure? Read More »

What different characteristic(s) exist between the two groups that account for the different staining conditions?

Experiment 3 Post-Lab Questions 1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points) Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall. Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin). 2. What

What different characteristic(s) exist between the two groups that account for the different staining conditions? Read More »

What color are the Gram-positive bacteria after Gram staining?

Experiment 3 Post-Lab Questions 1. What color are the Gram-positive bacteria after Gram staining? Gram-negative bacteria? (5 points) Gram-positive bacteria appear as dark purple or blue due to retaining the primary dye (Crystal Violet) in the cell wall. Gram-negative bacteria appear as red or pink due to decolorizing to accept the counterstain (Safranin). 2. What

What color are the Gram-positive bacteria after Gram staining? Read More »

What are some of the limitations of simple staining?

Experiment 1 Results Tables Table 1: Experiment 1 Staining Observations (5 points)     Stain used:   Crystal Violet Observations: Purple rod-shape bacteria with white background were observed         Experiment 1 Post-Lab Questions 1. How does crystal violet enhance the visualization of microbial features? (5 points) Crystal violet enhances the contrast between

What are some of the limitations of simple staining? Read More »

How does crystal violet enhance the visualization of microbial features?

Experiment 1 Results Tables Table 1: Experiment 1 Staining Observations (5 points)     Stain used:   Crystal Violet Observations: Purple rod-shape bacteria with white background were observed         Experiment 1 Post-Lab Questions 1. How does crystal violet enhance the visualization of microbial features? (5 points) Crystal violet enhances the contrast between

How does crystal violet enhance the visualization of microbial features? Read More »

what part of the cell do the antibiotics usually work?

Pre-Lab Questions 1. What determines if a bacterial cell is Gram-positive or Gram-negative? (5 points) Amount and location of the peptidoglycan molecule in the prokaryotic cell wall determines whether a bacterial cell is Gram-positive or Gram-negative.   2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research and describe

what part of the cell do the antibiotics usually work? Read More »

two antibiotics that affect Gram-negative bacteria?

Pre-Lab Questions 1. What determines if a bacterial cell is Gram-positive or Gram-negative? (5 points) Amount and location of the peptidoglycan molecule in the prokaryotic cell wall determines whether a bacterial cell is Gram-positive or Gram-negative.   2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research and describe

two antibiotics that affect Gram-negative bacteria? Read More »

On what part of the cell do the antibiotics usually work?

Pre-Lab Questions 1. What determines if a bacterial cell is Gram-positive or Gram-negative? (5 points) Amount and location of the peptidoglycan molecule in the prokaryotic cell wall determines whether a bacterial cell is Gram-positive or Gram-negative.   2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research and describe

On what part of the cell do the antibiotics usually work? Read More »

determine their class. Research and form a hypothesis on the evolutionary reasons for so many different bacterial morphologies

Pre-Lab Questions 1. What determines if a bacterial cell is Gram-positive or Gram-negative? (5 points) Amount and location of the peptidoglycan molecule in the prokaryotic cell wall determines whether a bacterial cell is Gram-positive or Gram-negative.   2. In this lab, both viruses and prions were introduced as acellular organisms. Do some research and describe

determine their class. Research and form a hypothesis on the evolutionary reasons for so many different bacterial morphologies Read More »