Effect of temperature on enzyme-catalyzed reactions
Through the first experiment-effect of temperature on enzyme-catalyzed reactions, we get temp (°C), absorbance and color changes of whole 5 sample with the use and compare of blank sample. No.1 sample is in 0 °C, the absorbance after whole experiment ends is 2.34 and it change to yellow, not original white. No.2 sample is in room temperature; the absorbance is 3.23 and it change to brown which is dark than yellow a little. No.3 sample is in 30°C, the absorbance is 3.48 and it change to dark-brown than normal brown a little. No.4 sample is in 40°C, the absorbance is 3.86 and it change to the brownest which near to color-black. No.5 sample is in 60°C, the absorbance is 1.51 and it change to obviously pink.
Table: Effect of temperature on extent of browning
| Sample | Temp (°C) | Absorbance | Any color changes? |
| Blank | Room temp | 0 | Clear |
| 1 | 0°C | 2.34 | Yellow |
| 2 | Room temp | 3.23 | Brown |
| 3 | 30°C | 3.48 | Dark-Brown |
| 4 | 40°C | 3.86 | Brownest |
| 5 | 60°C | 1.51 | Pink |
Graph: Effect of temperature on browning rate
2nd Experiment-Effect of PH on enzyme-catalyzed reactions
Through the second experiment-effects of pH on enzyme-catalyzed reactions, we get PH and absorbance of whole 5 sample with the use and compare of blank sample. No.1 sample with PH 3 and the absorbance is 1.76. No.2 sample with PH 5 and the absorbance is 1.08. No.3 sample with PH 7 and the absorbance is 2.98. No.4 sample with PH 9 and the absorbance is 0.51. No.5 sample with PH 11 and the absorbance is 0.98.
Table: Effect of PH on extent of browning
| Sample | PH | Absorbance |
| Blank | * | 0 |
| 1 | 3 | 1.76 |
| 2 | 5 | 1.08 |
| 3 | 7 | 2.98 |
| 4 | 9 | 0.51 |
| 5 | 11 | 0.98 |